Evaluación espectroscópica de la interacción entre la avenantramida-C presente en la avena y la albúmina sérica bovina
DOI:
https://doi.org/10.29105/cienciauanl27.124-4Keywords:
avenantramida-C, BSA, apagamiento de fluorescencia, polifenol, triptófano, avenaAbstract
Las avenantramidas son compuestos únicos presentes en la avena, han demostrado tener características antioxidantes, antiinflamatorias, anticancerígenas y neuroprotectoras. Sus propiedades benéficas se atribuyen a la interrelación con biomoléculas presentes en el organismo como lípidos y proteínas, por lo que el objetivo de este trabajo fue evaluar la interacción del principal compuesto fenólico en la avena, la avenantramida-C (Avn-C), y la albúmina sérica bovina (BSA) mediante espectroscopía de fluorescencia. Los resultados demuestran una disminución gradual en la intensidad de fluorescencia de la BSA conforme aumenta la concentración de Avn-C indicando la correlación y formación de un complejo Avn-C/BSA.
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Bose, A. (2016). Interaction of tea polyphenols with serum albumins: A fluorescence spectroscopic analysis, Journal of Luminescence, 169, 220-226. https://doi.org/10.1016/j.jlumin.2015.09.018 DOI: https://doi.org/10.1016/j.jlumin.2015.09.018
Bourassa, P., et al. (2010). Resveratrol, genistein, and curcumin bind bovine serum albumin, The Journal of Physical Chemistry B, 114(9), 3348-3354. https://doi.org/10.1021/jp9115996 DOI: https://doi.org/10.1021/jp9115996
Bratt, K., et al. (2003). Avenanthramides in oats (Avena sativa L.) and structure-antioxidant activity relationships, Journal of Agricultural and Food Chemistry, 51(3), 594-600. https://doi.org/10.1021/jf020544f DOI: https://doi.org/10.1021/jf020544f
Eberhardt, J., Santos-Martins, D., Tillack, A. F., et al. (2021). AutoDock Vina 1.2.0: New Docking Methods, Expanded Force Field, and Python Bindings. Journal of Chemical Information and Modeling, 61, 8, 3891-3898, https://doi.org/10.1021/acs.jcim.1c00203 DOI: https://doi.org/10.1021/acs.jcim.1c00203
Lakowicz, J.R. (2016) Principles of fluorescence spectroscopy. 3rd edn. New York: Springer. p. 954.
Ma, L., Maragos, C.M., y Zhang, Y. (2018). Interaction of zearalenone with bovine serum albumin as determined by fluorescence quenching, Mycotoxin Research, 34(1), 39-48, https://doi.org/10.1007/s12550-017-0297-7 DOI: https://doi.org/10.1007/s12550-017-0297-7
Quiroz-Vázquez, M.G., et al. (2020). Avenanthramide-C prevents amyloid formation of bovine serum albumin, Biophysical Chemistry, 263, 106391. https://doi.org/10.1016/j.bpc.2020.106391 DOI: https://doi.org/10.1016/j.bpc.2020.106391
Rasane, P. et al., (2013). Nutritional advantages of oats and opportunities for its processing as value added foods - a review, Journal of Food Science and Technology, 52(2), 662-675, https://doi.org/10.1007/s13197-013-1072-1 DOI: https://doi.org/10.1007/s13197-013-1072-1
Reinke, A.A., y Gestwicki, J.E. (2007). Structure-activity relationships of amyloid beta-aggregation inhibitors based on curcumin: Influence of linker length and flexibility, Chemical Biology and Drug Design, 70(3), 206-215, https://doi.org/10.1111/j.1747-0285.2007.00557.x DOI: https://doi.org/10.1111/j.1747-0285.2007.00557.x
Sang, S., y Chu, Y.F. (2017). Whole grain oats, more than just a fiber: role of unique phytochemicals, Molecular Nutrition & Food Research, 61(7), 1600715, https://doi.org/10.1002/mnfr.201600715 DOI: https://doi.org/10.1002/mnfr.201600715
Suryawanshi, V.D., et al. (2016). Spectroscopic analysis on the binding interaction of biologically active pyrimidine derivative with bovine serum albumin, Journal of Pharmaceutical Analysis, 6(1), 56-63, https://doi.org/10.1016/j.jpha.2015.07.001 DOI: https://doi.org/10.1016/j.jpha.2015.07.001
Wang, W., Snooks, H.D., y Sang, S. (2020). The chemistry and health benefits of dietary phenolamides, Journal of Agricultural and Food Chemistry, 1-57, https://doi.org/10.1021/acs.jafc.0c02605 DOI: https://doi.org/10.1021/acs.jafc.0c02605
Wu, W., et al. (2018). Avenanthramide aglycones and glucosides in oat bran: chemical profile, levels in commercial oat products, and cytotoxicity to human colon cancer cells, Journal of Agricultural and Food Chemistry, 66(30), 8005-8014. https://doi.org/10.1021/acs.jafc.8b02767. DOI: https://doi.org/10.1021/acs.jafc.8b02767